Producción de DNA extracelular en una estirpe natural de Bacillus subtilis

María Lamprecht Grandío

Departamento de Evolución Molecular

Año 2012

Share on facebook
Share on twitter
Share on linkedin

Extracellular DNA (eDNA) release is a widespread capacity described in many microorganisms. We identified and characterized lysis-independent eDNA production in an undomesticated strain of Bacillus subtilis. DNA fragments are released during a short time in late-exponential phase. The released eDNA corresponds to whole genome DNA, and does not harbour mutations suggesting that is not the result of error prone DNA synthesis. The absence of eDNA was linked to a spread colony morphology, which allowed a visual screening of a transposon library to search for genes involved in its production. Transposon insertions in genes related to quorum sensing and competence (oppA, oppF and comXP) and to DNA metabolism (mfd and topA) were impaired in eDNA release. Mutants in early competence genes such as comA and srfAA were also defective in eDNA while in contrast mutations in late competence genes as those for the DNA uptake machinery had no effect. A subpopulation of cells containing more DNA is present in the eDNA producing strains but absent from the eDNA defective strain. Finally, competent B. subtilis cells can be transformed by eDNA suggesting it could be used in horizontal gene transfer and providing a rationale for the molecular link between eDNA release and early- competence in B. subtilis that we report.

Datos de interés

Supervisores: José Eduardo González Pastor (CAB)
Universidad: Universidad Autónoma de Madrid. Facultad de Ciencias, Departamento de Biología Molecular; Centro de Astrobiología
Fecha de lectura: 17/12/2012